breeding Tag

02 Nov

Posted at 10:39h in Miscellaneous

रासायनिक उत्परिवर्तन द्वारा प्लांट ब्रीडिंग में टीलिंग और इको- टिलिंग की भूमिका TILLING (Targeting Induced Local Lesions in Genomes) is a method in molecular biology that allows directed identification of mutations in a specific gene or allele. TILLING was introduced in (McCallum et al. 2000) by using the model plant Arabidopsis thaliana. TILLING has since been used as a reverse genetics method in other organisms such as zebrafish, corn, wheat, rice, soybean, tomato and lettuce. TILLING is a general reverse genetic technique that combines chemical mutagenesis with PCR based screening to identify point mutations in regions of interest. TILLING is a powerful technology that employed heteroduplex analysis to detect which organism in a population carry single nucleotide mutation in specific genes. McCallum utilized reverse genetic approaches such as  T-DNA lines...

30 Nov

Posted at 05:01h in Crop Cultivation

गेहूँ में पूर्व-प्रजनन: संभावनाएं एवं चुनौतियां Pre‐breeding refers to all the activities designed to identify desirable characteristics and/or genes from un-adapted materials that cannot be used directly in breeding populations and to transfer these traits to an intermediate set of materials that breeders can use further in developing new varieties. Crop wild relatives have a high level of genetic diversity with rare alleles that enabled them to survive in natural and adverse environments. Most plant breeders fear in using exotic or un-adapted material due to its initial detrimental effects like linkage drag on elite breeding material. It is a necessary first step in the use of diversity arising from wild relatives and...