16 Nov Lateral flow immunoassay for detection of genetically modified crops
अनुवांशिक रूप से संशोधित फसलों का पता लगाने के लिए पार्श्व प्रवाह इम्यूनोएसे The development technologies in genetic engineering give rise to genetically modified organisms (GMOs). Genetically modified (GM) crops have become a reality in agriculture, food and feed market. Genetic engineering includes – insect resistance, herbicide tolerance, drought and salt tolerance, improved colors in fiber and flower crops, resistance to water logging and longer shelf-life. The genetically modified (gm) crops have contributed to the successful incorporation of different traits by introducing transgenes such as bacillus thuringiensis (bt) insecticidal genes (cry1ab, cry1ac, cry1f, cry2ab, cry3a, vip3), herbicide tolerant genes (cp4epsps, bar, pat, als), virus resistant (cp, prsv-cp, rep, hel), delayed ripening genes (sam-k, acc, pg), genes for color modification. B. thuringiensis and Cry protein toxins are efficient, safe and sustainable alternatives to chemical pesticides for the control of insect pests. The toxicity of the Cry proteins have traditionally been explained by the formation of trans membrane pores or ion channels that lead to osmotic cell lysis. The protein targetsthe major pests of cotton, tobacco, tomato, potato, corn, maize and rice.Mode of action of the crystal toxin on susceptible larvae involves ingestion of the crystalspore cell complex solubilization on th e midgut by the alkaline pH processing of the 51 and 42-kDa proteins to 43 and 39-kDa proteins binding of toxin proteins to cells of the gastric cecum and posterior midgut exertion of a toxic effect GM crops...