02 Nov Role of Tilling and Eco Tilling in Plant Breeding by Chemical mutagenesis
रासायनिक उत्परिवर्तन द्वारा प्लांट ब्रीडिंग में टीलिंग और इको- टिलिंग की भूमिका TILLING (Targeting Induced Local Lesions in Genomes) is a method in molecular biology that allows directed identification of mutations in a specific gene or allele. TILLING was introduced in (McCallum et al. 2000) by using the model plant Arabidopsis thaliana. TILLING has since been used as a reverse genetics method in other organisms such as zebrafish, corn, wheat, rice, soybean, tomato and lettuce. TILLING is a general reverse genetic technique that combines chemical mutagenesis with PCR based screening to identify point mutations in regions of interest. TILLING is a powerful technology that employed heteroduplex analysis to detect which organism in a population carry single nucleotide mutation in specific genes. McCallum utilized reverse genetic approaches such as T-DNA lines and antisense RNA, but was unable to successfully apply these approaches to characterize CMT2.The approach that was successful turned out to be what is now known as TILLING. TILLING can also be used to detect naturally occurring SNP in genes among the accession, variety or cultivar. To study the gene function, or to detect genetic marker in population. TILLING methodology can also be used to uncover natural nucleotide variation linked to important phenotypic characters, a process termed EcoTILLING (Comai et al. 2004). The current status of various plant TILLING and EcoTILLING methods are generally applicable across the plant kingdom. DNA sequence → Protein →...