Agriculture Knowledge for Everyone
Submit Article Contact Us
KrishiSewa - Agriculture Knowledge for Indian Farmers

micro propagation Tag

14 Jun

Posted at 13:50h in Crop Cultivation
Sidharth Sharma1 and Pankaj Kumar2,* 15 views

ऊतक  संवर्धन विधि द्वारा अखरोट (जुगलन्स रेजिया) का सूक्ष्म प्रसार Walnut seedling rootstocks have a very long juvenile period, while the seeds produced remain dormant for about 120 days. Although vegetative propagation through cuttings may be an option, this method is less viable, due to low rooting efficiency. जुगलन्स रेजिया, जिसे आमतौर पर फारसी अखरोट के रूप में जाना जाता है, मध्य एशिया का एक महत्वपूर्ण अखरोट फल है। हालांकि इसकी किस्मों की उपज क्षमता अलग-अलग होती है और दुनिया भर में व्यापक रूप से वितरित की जाती है, प्रजनकों और किसानों के सामने आने वाली समस्याएं काफी समान हैं। अखरोट सीडलिंग रूटस्टॉक्स की किशोर अवधि बहुत लंबी होती है, जबकि उत्पादित बीज लगभग 120 दिनों तक निष्क्रिय रहते हैं। हालाँकि, कलमों के माध्यम से वानस्पतिक प्रसार एक विकल्प हो सकता है, लेकिन कम जड़ने की दक्षता के कारण, यह विधि कम व्यवहार्य है। इन सभी समस्याओं को स्वीकार करते हुए, यह समीक्षा पुनर्जनन और प्रसार के क्षेत्र में किए गए शोध कार्यों और प्रयोगों के विश्लेषण पर केंद्रित है क्योंकि यह विधि यौन प्रजनन और भ्रूण के अंकुरण के चरण को लाँघ जाती है। एक सफल सूक्ष्मप्रवर्धन प्रसार विधि, चरणों की एक श्रृंखला के माध्यम से आगे बढ़ती है। ये हैं (i) असेप्टिक संवर्धन की शुरुआत, (ii) प्ररोह गुणन, (iii) सूक्ष्म शाखाओं का जड़...

24 May

Posted at 06:27h in Miscellaneous
Ram Niwas1*, Nidhika Rani1, Ankit Kumar Pandey2, Kumar Harsh2, Sampurna Nand Singh2, Gautam Pratap Singh3 and Ankur Kumar Rai2 16 views

सोमाक्लोनल वेरिएशन: फसल सुधार के लिए एक तकनीक Plant tissue culture procedures give a substitute strategy for vegetative propagation of horticultural crops. Clonal propagation through tissue culture (popularly known as micro propagation) can be acknowledged generally quickly inside a little space. The consistency of individual plants inside a clone population is a significant bit of choice of clonal cultivars in business creation. However, genetic variations do happen in undifferentiated cells, detached protoplasts, calli, tissues and morphological qualities of in vitro raised plants. In 1981, Larkin and Scowcroft begat an overall term ''somaclonal variation'' for plant variations got from any type of cell or tissue cultures. Somac1onal variation has been used in an enormous number of plant species such as potato, sugarcane, tobacco, tomato, wheat, rice, brassica and others for different agronomic qualities, for example, disease resistance, plant height, tiller number, maturity and for different physiological and biochemical characteristics. A few valuable somac1onal variations have been gotten and some of them have been released as cultivars (Hare et al. 2016). Somaclonal variation along these lines seems, by all accounts, to be a significant source of genetic fluctuation. Tissue culture-determined plants show variation which is similar to mutations in tissues and cultured cells. It has been discussed whether somaclonal variation is the consequence of previous genetic contrasts...

11 Mar

Posted at 07:11h in Seed production
Satish Kumar 15 views

फसल सुधार के लि‍ए फसलों में सूक्ष्मप्रजनन  Various methods of tissue culture and molecular biology have shown tremendous potential for creation, conservation and precise characterization of genetic variability for improvement of fruit, field, forest crops, medicinal and aromatic plants. Micropropagation of crops is now one of the best and most successful examples of the commercial application of tissue culture technology. Propagation of plants from very small plant tissues/ parts under in vitro conditions in the laboratory is called micropropagation. Commercial application of tissue culture started in 1970 in USA with the micropropagation of orchids. Since then it has seen tremendous expansion globally both in terms of number of production units and the number of plants, as well. With the estimated global market of 15 billion US$ per annum for tissue cultured products, even with the exponential expansion in the industry, the demand far exceeds production, leaving enough scope for expansion. Due to higher labour costs in the developed countries, micropropagation industry is now being expanded in the developing countries, with lower wage scales to multiply plants at much cheaper rates even for the international markets. Thus Indian micropropagation industry, though a late starter by almost a decade, has expanded exponentially from 5 million plants annual capacity in 1988 to around 200...